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KMID : 0382619860060020071
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Hanyang Journal of Medicine
1986 Volume.6 No. 2 p.71 ~ p.83
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Fractionation and Characterization of proteins and Ribonucleases in amniotic fluid
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Abstract
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Changes in protein contents and neutral ribonuclease (RNase) activities in human amniotic fluid with gestational weeks were investigated. Proteins and RNases in amniotic fluid were separated and fractionated by polyacrylamide gel electrophoresis (PAGE) and DEAE-cellulose column chromatography to observe how the electrophoresic and chromatographic patterns of the proteins and enzymes were changed with gestational age.
1. Protein contents and neutral RNase activities in amniotic fluid were decreased with increase in weeks of gestation.
2. Proteins in amniotic fluid at 20 weeks of gestation were separated into 8 bands by SDS-PAGE, and some of the protein bands disappeared in the amniotic fluid with increase in weeks of gestation.
3. DEAE-cellulose column chromatographical analysis for amniotic fluid proteins revealed that proteins in amniotic fluid were fractionated into 5 peaks, and that protein peak II decreased and peak III incresed in the amniotic fluid with weeks of gestation.
4. Neutral RNases in amniotic fluid were also fractionated into 5 peaks by a
DEAE-cellulose column chromatography, and RNase peak II increased
and peak III decreased in the amniotic fluid with weeks of gestation.
The results obtained in the present study suggested that understanding in changes with gestational week in amniotic fluid protein contents and neutral RNase activities and in protein and RNase patterns obtained by PAGE and DEAE-cellulose column chromatography could possibly be used as diagnostic markers for fetal maturation and intrauterine growth retardation.
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